disc1  (Santa Cruz Biotechnology)

Journal: Life Science Alliance

Article Title: Kalirin-RAC controls nucleokinetic migration in ADRN-type neuroblastoma

doi: 10.26508/lsa.201900332

Figure Lengend Snippet: (A) Immunolabelling of kalirin in IMR-32 after KALRN RNAi. Scale bar 20 μm. (B) Western blot analysis of kalirin-8 and kalirin-STYV levels in IMR-32 and SK-N-BE(2)c after KALRN RNAi and kalirin-8 levels in IMR32 after KALRN RNAi or additional negative control siRNAs: control siRNA #2, HNRNPK siRNA, and DISC1 siRNA. (C) RAC1 activity in IMR-32 and SK-N-BE(2)c cell treated with kalirin-GEF1 inhibitor#1 (10 μM), kalirin-GEF1 inhibitor#2 (5 μM), RHOA inhibitor (3 μM), RAC1 inhibitor (10 μM), or after SOX11 and KALRN RNAi. (D) Kalrn expression in t-SNE-resolved E12.5 and E13.5 sympathetic precursors: sympathoblasts, Schwann cell precursors, bridge population and chromaffin cells . (E) Sequencing electrophoregrams showing 3′-UTR of kalirin-9 isoform (top) and the exon scheme based on the results of sequencing (bottom). Location of stop codon is marked with “-.” (F) KALRN , TRIO , and TIAM expression in SK-N-BE(2)c (left) after RA (10 μM) treatment. x-axis indicate timepoints in hours. Western Blot analysis of kalirin, TRIO, and βIII-tubulin in SK-N-BE2c after 72 h of RA-treatment (top right) and of TRIO and kalirin-8 in IMR-32 and SK-N-BE(2)c after 72 h of RA treatment (bottom right). (G) Cell viability of NB cell lines treated with vehicle, kalirin-GEF1 inhibitor#2, or kalirin-GEF1 inhibitor#1. Values are reported as mean percent ± SD of vehicle-treated control.

Article Snippet: KALRN (sc-18592), LIS1 (sc-35814), DCX (sc-35214), HNRNPK (sc-38282), and DISC1 (sc-60539) were purchased from Santa Cruz Biotechnologies.

Techniques: Western Blot, Negative Control, Control, Activity Assay, Expressing, Sequencing

Journal: Oncotarget

Article Title: DISC1 overexpression promotes non-small cell lung cancer cell proliferation

doi: 10.18632/oncotarget.18055

Figure Lengend Snippet: DISC1 and PCNA levels in eight paired NSCLC tumor (T) and adjacent non-tumor (N) tissues as shown by western blot analysis (A) GAPDH was used as a loading control. DISC1 (B) and PCNA (C) levels were analyzed in tumor tissues (normalized to GAPDH). * P <0.05, compared with adjacent non-tumor tissues. DISC1 expression in NSCLC cell lines (A549, H1299, and SPCA-1) as shown by western blot analysis (D) GAPDH was used as a loading control. DISC1levels were analyzed in NSCLC cell lines (normalized to GAPDH) (E).

Article Snippet: siRNAs targeting DISC1 and negative control siRNA (NC-siRNA) were purchased from GenePharma Co., Ltd (China) (Table ), and a DISC1 over-expression plasmid (DISC1-Flag) (GeneCopoeia, China) was also used. siRNAs and plasmid were transfected into NSCLC cell lines using Lipofectamine 2000 (Life Technologies, USA) according to the manufacturer’s instructions.

Techniques: Western Blot, Control, Expressing

Journal: Oncotarget

Article Title: DISC1 overexpression promotes non-small cell lung cancer cell proliferation

doi: 10.18632/oncotarget.18055

Figure Lengend Snippet: IHC staining of DISC1 (A) , p-GSK3β (B) , β-catenin (C) , Cyclin D1 (D) , and Ki-67 (E) in well- (1), moderately- (2), and poorly-differentiated (3) NSCLC tissues (200×).

Article Snippet: siRNAs targeting DISC1 and negative control siRNA (NC-siRNA) were purchased from GenePharma Co., Ltd (China) (Table ), and a DISC1 over-expression plasmid (DISC1-Flag) (GeneCopoeia, China) was also used. siRNAs and plasmid were transfected into NSCLC cell lines using Lipofectamine 2000 (Life Technologies, USA) according to the manufacturer’s instructions.

Techniques: Immunohistochemistry

Journal: Oncotarget

Article Title: DISC1 overexpression promotes non-small cell lung cancer cell proliferation

doi: 10.18632/oncotarget.18055

Figure Lengend Snippet: DISC1 expression correlated with that of p-GSK3β (A) , β-catenin (B) , Cyclin D1 (C) , and Ki-67 (D) .

Article Snippet: siRNAs targeting DISC1 and negative control siRNA (NC-siRNA) were purchased from GenePharma Co., Ltd (China) (Table ), and a DISC1 over-expression plasmid (DISC1-Flag) (GeneCopoeia, China) was also used. siRNAs and plasmid were transfected into NSCLC cell lines using Lipofectamine 2000 (Life Technologies, USA) according to the manufacturer’s instructions.

Techniques: Expressing

Journal: Oncotarget

Article Title: DISC1 overexpression promotes non-small cell lung cancer cell proliferation

doi: 10.18632/oncotarget.18055

Figure Lengend Snippet: Associations between DISC1 expression and NSCLC patient clinicopathological parameters

Article Snippet: siRNAs targeting DISC1 and negative control siRNA (NC-siRNA) were purchased from GenePharma Co., Ltd (China) (Table ), and a DISC1 over-expression plasmid (DISC1-Flag) (GeneCopoeia, China) was also used. siRNAs and plasmid were transfected into NSCLC cell lines using Lipofectamine 2000 (Life Technologies, USA) according to the manufacturer’s instructions.

Techniques: Expressing

Journal: Oncotarget

Article Title: DISC1 overexpression promotes non-small cell lung cancer cell proliferation

doi: 10.18632/oncotarget.18055

Figure Lengend Snippet: Univariate analysis of NSCLC patient clinicopathological parameters with respect to survival

Article Snippet: siRNAs targeting DISC1 and negative control siRNA (NC-siRNA) were purchased from GenePharma Co., Ltd (China) (Table ), and a DISC1 over-expression plasmid (DISC1-Flag) (GeneCopoeia, China) was also used. siRNAs and plasmid were transfected into NSCLC cell lines using Lipofectamine 2000 (Life Technologies, USA) according to the manufacturer’s instructions.

Techniques: Expressing

Journal: Oncotarget

Article Title: DISC1 overexpression promotes non-small cell lung cancer cell proliferation

doi: 10.18632/oncotarget.18055

Figure Lengend Snippet: Cox regression analysis of potential prognostic factors with respect to NSCLC patient survival

Article Snippet: siRNAs targeting DISC1 and negative control siRNA (NC-siRNA) were purchased from GenePharma Co., Ltd (China) (Table ), and a DISC1 over-expression plasmid (DISC1-Flag) (GeneCopoeia, China) was also used. siRNAs and plasmid were transfected into NSCLC cell lines using Lipofectamine 2000 (Life Technologies, USA) according to the manufacturer’s instructions.

Techniques: Expressing

Journal: Oncotarget

Article Title: DISC1 overexpression promotes non-small cell lung cancer cell proliferation

doi: 10.18632/oncotarget.18055

Figure Lengend Snippet: Cumulative overall NSCLC patient survival rate was associated with high (green line) or low (blue line) DISC1 expression.

Article Snippet: siRNAs targeting DISC1 and negative control siRNA (NC-siRNA) were purchased from GenePharma Co., Ltd (China) (Table ), and a DISC1 over-expression plasmid (DISC1-Flag) (GeneCopoeia, China) was also used. siRNAs and plasmid were transfected into NSCLC cell lines using Lipofectamine 2000 (Life Technologies, USA) according to the manufacturer’s instructions.

Techniques: Expressing

Journal: Oncotarget

Article Title: DISC1 overexpression promotes non-small cell lung cancer cell proliferation

doi: 10.18632/oncotarget.18055

Figure Lengend Snippet: DISC1 was silenced using siRNAs (DISC1-siRNA1, DISC1-siRNA2 and DISC1-siRNA3) in A549 cells, as detected by western blotting (A) DISC1 knockdown by DISC1-siRNA1, DISC1-siRNA2, and DISC1-siRNA3 (B) PCNA and Cyclin D1 levels were detected by western blotting (C) DISC1-siRNA2-treated A549 cell proliferation as measured by CCK-8 assay (D) Colony formation by DISC1-siRNA2-treated A549 cells (E) The number of cell clones by DIC1-siRNA2-treated A549 cells (F) * P <0.05 compared NC-siRNA-treated cells.

Article Snippet: siRNAs targeting DISC1 and negative control siRNA (NC-siRNA) were purchased from GenePharma Co., Ltd (China) (Table ), and a DISC1 over-expression plasmid (DISC1-Flag) (GeneCopoeia, China) was also used. siRNAs and plasmid were transfected into NSCLC cell lines using Lipofectamine 2000 (Life Technologies, USA) according to the manufacturer’s instructions.

Techniques: Western Blot, Knockdown, CCK-8 Assay, Clone Assay

Journal: Oncotarget

Article Title: DISC1 overexpression promotes non-small cell lung cancer cell proliferation

doi: 10.18632/oncotarget.18055

Figure Lengend Snippet: After DISC1 inhibition or upregulation in A549 cells using DISC1-siRNA2 or DISC1-Flag, respectively, DISC1, p-GSK3β, β-catenin, and cyclin D1 were detected via western blotting.

Article Snippet: siRNAs targeting DISC1 and negative control siRNA (NC-siRNA) were purchased from GenePharma Co., Ltd (China) (Table ), and a DISC1 over-expression plasmid (DISC1-Flag) (GeneCopoeia, China) was also used. siRNAs and plasmid were transfected into NSCLC cell lines using Lipofectamine 2000 (Life Technologies, USA) according to the manufacturer’s instructions.

Techniques: Inhibition, Western Blot

Journal: Oncotarget

Article Title: DISC1 overexpression promotes non-small cell lung cancer cell proliferation

doi: 10.18632/oncotarget.18055

Figure Lengend Snippet: Hypothesized mechanism for DISC1 overexpression promotion of NSCLC cell proliferation

Article Snippet: siRNAs targeting DISC1 and negative control siRNA (NC-siRNA) were purchased from GenePharma Co., Ltd (China) (Table ), and a DISC1 over-expression plasmid (DISC1-Flag) (GeneCopoeia, China) was also used. siRNAs and plasmid were transfected into NSCLC cell lines using Lipofectamine 2000 (Life Technologies, USA) according to the manufacturer’s instructions.

Techniques: Over Expression

Journal: Oncotarget

Article Title: DISC1 overexpression promotes non-small cell lung cancer cell proliferation

doi: 10.18632/oncotarget.18055

Figure Lengend Snippet: DISC1-targeting siRNAs

Article Snippet: siRNAs targeting DISC1 and negative control siRNA (NC-siRNA) were purchased from GenePharma Co., Ltd (China) (Table ), and a DISC1 over-expression plasmid (DISC1-Flag) (GeneCopoeia, China) was also used. siRNAs and plasmid were transfected into NSCLC cell lines using Lipofectamine 2000 (Life Technologies, USA) according to the manufacturer’s instructions.

Techniques: Sequencing